I put in an Eclipse Radio and the Nav calls for a VSS input. As I dont have a service manual, can anyone tell me where to pick up a VSS signal on a 2001 Tundra?

You can get the wiring diagrams for your truck in the Knowledge Base forums. It's at the bottom of the main page. Click on the Vehicle Specific link under the Subscriber Requests, Discussions and Technical Assistance link. After clicking on the Vehicle Specific link, scroll down to the 2001 Tunda. Click that and scroll down until you find the 2001 Tundra Electrical Wiring Diagram link. That will give a bunch of different pdf files with for different wiring diagrams. The wire you're looking for should be a green wire with an orange stripe coming off of one of the plugs on the back of your gauge cluster. You can get the reverse wire on one of those plugs also. There's also a pdf file in there to show you what the different plugs look like and how their numbered so you can find the wire. Take a little while and learn how to read the wiring diagrams. The two files you'll use are the combination meter file and the connector list file. The various knowledge base files have a ton of information and are very useful.